You create a recombinant DNA molecule that fuses the coding sequence of green fluorescent protein to the regulatory DNA sequences that control the expression of your favorite genes
Which of the following pieces of information can you NOT gain by examining the expression of this reporter gene?
(a) the tissue where the protein encoded by this gene is expressed
(b) the cell in which the protein encoded by this gene is expressed
(c) the specific location within the cell of the protein encoded by this gene
(d) when, during an organism's development, this gene is expressed
(c) The information for localizing proteins within a cell is found on the protein product and not in the regulatory DNA sequences. If you were to fuse your reporter gene to the DNA sequences that encode the protein to produce a GFP fusion protein, then you might determine the specific location of the protein within the cell.
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Which type of regulator(s) specifically bind to operator regions of DNA?
A) activators B) activators and inducers C) repressors D) repressors and corepressors
You want to identify Mycobacterium tuberculosis in a patient sputum sample. Which procedure would be most useful in this case?
A. Polymerase chain reaction B. Whole genome sequencing C. Microarray analysis D. DNA probe analysis
For a given cross, the expected number of double recombinants is 20 and the observed number of double recombinants is 15. What is the interference calculation (I)?
A) 0.25 B) 1.33 C) 20 D) 0.75 E) 15
Sometimes, the decline of one species threatens the survival of another species. Which scenario is an example of this?
A. Declines in polar bears result in diminished prey populations B. The arrival of sailors on Mauritius island led to the extinction of dodo birds C. Declines in eagle populations due to the accumulation of pollutants in their eggs D. Decline in buffalo caused a drop in native buffalo clover E. Pollution led to declines in the Texas blink salamander population