Assume that the gene trpA in an auxotrophic strain of E. coli is located at 27 minutes, whereas the gene pyrE is located at 81 minutes
Present an experimental scheme that would allow you to convert one of the auxotrophic strains to a prototrophic strain.
A) F+ (auxotroph) × F? (wild type)
B) F+ (wild type) × F? (auxotroph)
C) Hfr (auxotroph) × F? (auxotroph)
D) Hfr (wild type) × F? (auxotroph)
E) Hfr (auxotroph) × F? (wild type)
Answer: D
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