You cut some sheep DNA and some plasmid DNA with the same restriction enzyme. The plasmid contains an AmpR gene and a lacZ gene. You mix together the fragments of sheep DNA and the linearized plasmid. You transform bacteria with your mixture, and plate the bacterial cells on growth media containing the antibiotic ampicillin and X-gal (the substrate of beta-galactosidase). You need to pick some bacterial colonies that contain recombinant plasmids. Which colonies do you choose?
A. any colony; if they grow on the media they are recombinant.
B. the white colonies; only cells that contain a recombinant plasmid will be white.
C. the blue colonies; any cells containing eukaryotic (sheep) DNA will be blue.
D. the large colonies; large colonies are more resistant to the antiobiotic and thus more likely to contain a recombinant plasmid.
E. any colony, all colonies will contain plasmids; it requires additional testing to determine if the plasmid is recombinant.
B. the white colonies; only cells that contain a recombinant plasmid will be white.
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