The human gene for a protein hormone has been isolated. Scientists want to introduce this gene into a bacterial host, modifying the bacteria so that it can be used to produce this hormone. After successfully introducing the gene on a plasmid, the bacteria produced the protein hormone, but the protein was defective, longer than normal, with intervening stretches of amino acids not found in the native protein. Which solution would be most likely to resolve this problem?
A. Treat the defective protein with enzymes.
B. Start over, introducing a complementary DNA (cDNA) copy of the gene into the bacterial host.
C. Introduce human ribosomes into the bacteria.
D. Introduce human mRNAs into the bacterial host.
Answer: B
You might also like to view...
A well-substantiated explanation of some aspect the natural world, based on a body of facts that have been repeatedly confirmed through observations and experiments, is referred to as a(n) ________.
A. hypothesis B. theory C. fact D. idea
Which of the following is mostly performed by bacteria living within the roots of plants in the legume family?
a. nitrification b. ammonification c. nitrogen cycling d. nitrogen fixation e. nitrogen assimilation
In gel electrophoresis, DNA fragments move at different rates because they have different
A) electrical charge densities. B) sizes. C) base sequences. D) amino acid compositions. E) overall electrical charges.
The comparative anatomist Georges Cuvier confirmed the concept of extinction in 1812 when he demonstrated that there were no extant species anatomically related to the fossilized remains of the ________
A) mastodon B) Irish elk C) pygmy armadillo D) giant vampire bat E) Arctic lemming