You are given the task of restriction-mapping a portion of a 10 kb linear genome fragment from a virus. You digest the fragment with BamHI and, upon gel electrophoresis, observe bands corresponding to 4 kb, 5 kb and 1 kb
When you digest the fragment with the enzyme HindII, you observe bands corresponding to 4 kb and 3 kb. When you digest the 10 kb fragment with both enzymes, you observe bands corresponding to 3 kb, 2 kb, and 1 kb, although the 2 kb band is much brighter than the other two bands. Based on this information, draw the restriction map of the 10 kb fragment.
What will be an ideal response?
Answer: B B
_______|___________________|_________________________
1 kb 4 kb 5 kb
H H
________________|___________________|_________________
3 kb 4 kb 3 kb
B H B H
_______|_________|__________|_________|________________
1 kb 2 kb 2 kb 2 kb 3 kb
B = BamHI restriction site
H = HindII restriction site
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