A student carries out PCR using the following steps: Step 1: 94°C for 1 minute Step 2: 60°C for 30 seconds Step 3: 72°C for 30 seconds Which of the following lists the CORRECT terms for these three steps?
A. denaturation of the double-stranded template, extension of the new DNA molecules, primer annealing
B. degradation of the template, primer annealing, extension of the new DNA molecules
C. denaturation of the double-stranded template, primer annealing, extension of the new DNA molecules
D. denaturation of the double-stranded template, extension of the new DNA molecules, hybridization of the template
E. hybridization of the single-stranded templates, primer annealing, extension of the new DNA molecules
Ans: C. denaturation of the double-stranded template, primer annealing, extension of the new DNA molecules
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