You have joined a lab studying DNA replication in E. coli. The graduate student you are working with has identified a mutation in primase that makes primase very inefficient. Your project is to characterize the cells with this mutation. Predict the defects you would most likely see in the mutant E. coli cells.
A. inefficient Okazaki fragment joining
B. a longer total time to replicate DNA
C. a delay in the unwinding of DNA
D. a delay in DNA polymerase beginning synthesis
E. rapid lagging-strand synthesis but slow leading-strand synthesis
Answer:
B. a longer total time to replicate DNA
D. a delay in DNA polymerase beginning synthesis
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