MacConkey agar serves which purpose when used in the bacterial fecal culture?
a. Evaluation of general flora and predomi-nant species
b. Selection of enteric pathogens such as Salmonella, Aeromonas, and others
c. Inhibition of normal flora and selection of enteric pathogens
d. Enrichment for enteric pathogens such as Salmonella and others
B
The slightly selective agar should support growth of most Enterobacteriaceae, Vibrio, and other possible pathogens; MacConkey agar works well. Some laboratories use eosin-methylene blue (EMB), which is slightly more inhibitory. All lactose-negative colonies should be tested further, ensuring adequate detection of most vibrio and most pathogenic Enterobacteriaceae. Lac-tose-positive vibrio (Vibrio vulnificus), pathogenic E. coli, some Aeromonas spp., and Plesi-omonas spp. may not be distinctive on MacConkey agar.
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A. endospores are significantly smaller than vegetative cells. B. endospores are resistant to harsh environments, thus allowing survival of endospore-forming organisms under conditions in which non-endospore-forming cells would not survive, and endospore-forming organisms are often dangerous pathogens. C. they are resistant to harsh environments, thus allowing survival of endospore-forming organisms under conditions in which non-endospore-forming cells would not survive. D. endospore-forming organisms are often dangerous pathogens.
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