What enables a PCR-amplified gene to be inserted into a cloning vector?
a. The PCR reaction adds sticky ends onto the amplified DNA, allowing ligation to a cloning vector.
b. Restriction enzyme sites are added to the 5? ends of both primers; therefore, digestion of the PCR-amplified
gene allows ligation to a cloning vector cut with the same restriction enzyme.
c. The PCR-amplified gene is sequenced to locate restriction sites in order to determine which restriction
enzymes to use for cloning.
d. The PCR-amplified gene is cut with one restriction enzyme, then ligated to a cloning vector cut by a different
restriction enzyme.
e. The cloning vector is added to the PCR reaction, and once the gene is amplified, it immediately ligates to the
cloning vector.
ANSWER: b
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What is the main difference between monotremes and eutherians?
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