What is the main reason for using a cDNA library rather than a genomic library to isolate a human gene from which you wish to make large quantities of the human protein in bacteria?

What will be an ideal response?


The gene isolated from a genomic library would still contain introns, and bacteria do not contain the biochemical machinery for removing introns by RNA splicing. The same gene isolated from the cDNA library will already have had its introns removed.

Biology & Microbiology

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Robert Koch and his colleagues first used agar in bacteriological media.  Why was this ingredient an improvement on previous materials used to culture bacteria?

A. Bacteria growing on the solidified medium did not degrade or break down the agar. B. Agar can convert from liquid to solid form, and back again, very easily. C. Agar is a complete source of nutrients for most bacteria. D. Agar was less expensive than the nutrients that Koch was previously using in the lab.

Biology & Microbiology

A plant grown at high density will produce ________ seeds than the same plant grown at low density

Fill in the blank(s) with correct word

Biology & Microbiology

During the late winter, maple tree sap starts to flow from the roots towards the branches of a tree. Maple sap is composed of water and sugars. How is it possible for a maple tree to have glucose available in sap when the tree does not have leaves on its branches?

A) Glucose is produced in the roots and transferred throughout the tree during late winter. B) Glucose is stored in the roots over winter and flows within the tree when leaves and buds start to form. C) Glucose is absorbed by the root systems and can be first absorbed in late winter as the ground starts to thaw. D) Glucose is still produced during winter within the bark of the tree.

Biology & Microbiology

The C-value paradox is:

a. the incongruity of genome size with organismal complexity. b. resolved with the identification of noncoding DNA. c. the incongruity of genome size with noncoding DNA. d. A and B. e. All of the above.

Biology & Microbiology