A manual hemoglobin is determined on a patient, and the dilution still appears turbid after letting it sit for 15 minutes. The stained blood film is examined, and sickle cells are observed on the blood film
Equal volumes of the diluted hemoglobin sample and water are mixed together, and the absorbance of this read on a spectrophotometer. How should the hemoglobin result be determined from the standard calibrated graph?
a. Add the results from the turbid and second dilution together and divide by 2.
b. Multiply the result obtained from the graph on the diluted specimen by 2.
c. Divide the result obtained from the graph on the diluted specimen by 2.
d. There was no reason to perform this second dilution.
ANS: B
Red cells that contain Hb S (sickle hemoglobin) are resistant to lysis in some cyanmethemoglobin reagents. When this occurs, the cyanmethemoglobin dilution should be mixed with an equal part of water, and the optical density (percent transmission) read. This optical density reading is read from the calibration graph to obtain the hemoglobin result. However, because the diluted specimen was diluted 1:2 by water, this result must be multiplied by 2 to obtain the patient's result.
You might also like to view...
The phlebotomist has a 72-year-old patient named Helga Schmidt. How should the phlebotomist address this patient?
A. Ms. Schmidt B. Frau Schmidt C. Helga D. Honey
You have just assisted in the delivery of a 34-week-gestation infant in the office of a methadone clinic. After 30 seconds of drying and stimulation, the infant remains limp and cyanotic, with a pulse of 50. Which of the following is the most appropriate action to take at this point?
A) Begin CPR at a rate of 3 compressions to 1 ventilation.
B) Intubate the trachea, ventilate, reassess, and prepare to transport to a NICU.
C) Begin bag-valve-mask ventilations and establish intravenous access.
D) Continue to warm and dry the infant for an additional 30 seconds, while preparing to intubate.
You are an EMR in a rural county of 450 square miles that has two centrally located transporting ambulances. Which one of the following would be an MCI in this setting?
A) A motor vehicle collision with eight patients from two vehicles B) An exposure to chemical vapors with three critical and two stable patients C) A day care center fire with 12 minor injuries D) All of the above
How does moving a spontaneously breathing person from a horizontal supine position to the prone or lateral decubitus position influence the FRC?
A. The FRC increases from 15% to 20%. B. The FRC increases from 30% to 50%. C. The FRC decreases from 20% to 15%. D. The FRC decreases from 50% to 30%.