Under what circumstance might slow-growing mycobacteria appear to produce colonies in a few days?

a. When the inoculum is too heavy
b. When subcultured to an enriched medium
c. When incubated at slightly warmer tem-peratures
d. When kept in the light


A
The rate of growth is an important criterion for determining the initial category of an isolate. The dilution of the organism used to assess the growth rate is critical. Slow-growing mycobacteria appear to produce colonies in less than 7 days if the inoculum is too heavy. Rapid-growers usually produce colonies within 3 to 4 days after subculture. Even a rapid-grower, however, may take longer than 7 days to produce colonies initially because of the inhibition by a harsh decon-taminating procedure. Therefore the subculture must determine the growth rate and pigment production.

Biology & Microbiology

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