You expose a tube of bacteria to ultraviolet radiation to induce mutations. You then spread the bacteria on a plate containing media with all known required nutrients and wait until separate colonies form. Finally, you use replicate plating to transfer a print of this plate to an additional sterile plate containing minimal media plus a known required nutrient for the bacteria. Based on this protocol, colonies growing on the plate with minimal media would include what type of cells?

A. only mutant bacteria
B. mutants that only require the supplied nutrient
C. Both wild-type and all mutants
D. only wild-type bacteria
E. wild-type bacteria and the mutants that only require the supplied nutrient


Answer: E

Biology & Microbiology

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