Your research project involves the characterization of a recently identified transcription factor. As part of your project, you want to determine if this transcription factor binds directly to any of the general transcription factors. Unfortunately, you are having trouble expressing the full-length protein. What approach may help achieve your research goal?
A. You must find a way to express the full-length protein, since transcription factor functions do not lie in separate domains.
B. Express and purify only the DNA-binding domain, and test for binding.
C. Express and purify only the activation domain, and test for binding.
D. Identify the RNA that mediates the protein-protein binding.
Answer: C
You might also like to view...
In E. coli, lactose binds with:
A. the lactose operon repressor, preventing it from binding to the operator. This allows the lacZ and lacY genes to be transcribed. B. the lactose operon operator, preventing it from binding to the repressor. This allows the lacZ and lacY genes to be transcribed. C. the lactose operon repressor, helping it bind to the operator. This prevents the lacZ and lacY genes from being transcribed. D. the lactose operon operator, preventing RNA polymerase from being recruited. This prevents the lacZ and lacY genes from being transcribed. E. the lactose operon operator, recruiting RNA polymerase. This allows the lacZ and lacY genes to be transcribed.
Which of the following is NOT true of
polysaccharides? a. composed of many linked monosaccharides b. may be straight and unbranched or highly branched c. most likely made of glucose molecules if they are one of the natural polysaccharides d. may be insoluble because of their large size and structure e. are formed by cleavage reactions
Which of the following is currently used by the U.S. Department of Justice as a form of genetic "fingerprints" of criminals?
A) PCRs B) The genes responsible for producing the unique patterns of swirls and loops on a person's fingers C) STRs D) Plasmids E) The number of introns in a chromosome
What type of cell retains the ability to divide repeatedly into any type of cell, thus allowing the body to repair and grow?
What will be an ideal response?