List at least five criteria for quality control and assurance of FISH procedures
What will be an ideal response
1. Technologists should agree on blinded specimens within 5%.
2. Mosaicism should be determined on two independently processed slides.
3. Reporting criteria must be established independently for each probe type.
4. Interphase cells must be intact with smooth, nonoverlapping margins.
5. Slides with excess nonspecific staining should be repeated.
6. Only metaphase cells should be read for whole chromosome paints.
7. Probes should be validated using internal sequences in the target and external sequences in known positive and
negative controls.
8. Probe sensitivity and specificity should approximate 98%.
9. Diagnosis with interphase cells requires a minimum of 50 cells (although some probes require up to 500 ), and
diagnosis with metaphase cells requires a minimum of 5–20 cells.
10. A minimum of two pictures must be taken of each specimen for permanent records.
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A. prevention education
B. a drug-free lifestyle
C. the maintenance of "balance"
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