Probes used for detecting genetic sequences are frequently composed of
A) agarose.
B) synthetic nucleic acids and labeled conjugates, such as fluorescent dyes.
C) restriction enzymes.
D) silicon chips.
E) nitrocellulose.
B
Bloom's Taxonomy: Application
Section: The Tools of Recombinant DNA Technology
Learning Outcome: 8.7
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If we are only able to observe a population’s phenotypes, which frequencies of genotypes can we know?
a. both homozygous dominant and recessive b. only homozygous dominant c. only homozygous recessive d. both homozygous and heterozygous
Antigens interact with antibodies at
A. the outer end of each arm of the Y. B. the junction of heavy and light chains. C. different regions depending on the class of antibody. D. the bottom stem of the heavy chain of the Y.
Which of the following events must occur to cause a chromosomal translocation?
A. One chromosome must break in two distinct locations. B. Two different chromosomes must break. C. A transposon must be present in one of the chromosomes. D. The broken ends must pair correctly. E. A mutation in the DNA must occur.
Isothermal amplification techniques, such as NASBA, utilize a single temperature for nucleic acid amplification thereby eliminating the need for thermal cycling instruments to precisely and quickly alter the reaction temperature. This often reduces the cost associated with molecular testing and infrastructure requirements. In what settings could this be advantageous?
a. Developing countries with limited health care facilities b. Field clinics during military operations c. Small clinics or hospitals without centra-lized laboratory facilities d. Urgent care clinics or pharmacies for point-of-care diagnosis e. All of the above