How can the correct platelet count on a patient be determined when his or her platelets repeatedly clump in an EDTA specimen?
a. Re-collect in sodium citrate tube (blue top) and multiply by 0.5.
b. Re-collect in sodium citrate tube (blue top) and multiply count by 1.1.
c. Report the estimated number from the blood film.
d. Re-collect in an EDTA tube.
ANS: B
When platelet clumping repeatedly occurs in an EDTA blood, the blood should be drawn in a sodium citrate tube. The dilution of blood by the anticoagulant is 9:10 (9 parts blood to 1 part anticoagulant). The platelet count obtained on this tube must therefore be multiplied by the reciprocal of the dilution (10/9) = 1.1.
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