A scientist edits a mammalian genome by inserting a GFP reporter sequence downstream of a gene of interest. Which of the following methods is she likely to have used to make this change?

A. site-directed mutagenesis
B. CRISPR-Cas9 genome editing followed by nonhomologous end joining (NHEJ)
C. mutagenesis with radiation
D. CRISPR-Cas9 genome editing followed by homologous recombination (HR) with a donor template
E. oligonucleotide-directed mutagenesis


Ans: D. CRISPR-Cas9 genome editing followed by homologous recombination (HR) with a donor template

Biology & Microbiology

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The energy sources needed for the light reactions and the carbon reactions, respectively, are

A. Sunlight serves as the energy source for both sets of photosynthetic reactions. B. ATP and carbon dioxide. C. sunlight and ATP. D. ATP and sunlight. E. sunlight and carbon dioxide.

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The first commercial product made with recombinant DNA techniques was ____________________. Fill in the blank(s) with the appropriate word(s)

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Identify which is a symptom from the list of signs below.

A) fever B) leukopenia C) nausea D) dropping blood pressure

Biology & Microbiology