What is polycistronic mRNA?
What will be an ideal response?
Polycistronic mRNA is seen primarily among prokaryotes in which one mRNA carries coding information and internal punctuation for the translation of more than one protein.
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The frequency of heterozygotes for cystic fibrosis shows ethnic variations. Indicate whether the statement is true or false
A specific stretch of DNA that programs the amino acid sequence of a polypeptide is a ________
A) gene B) protein C) nucleic acid D) enzyme
A polymerase chain reaction (PCR) copies an individual gene segment in vitro with a(n) ________ primer(s)
A) individual RNA B) individual DNA C) pair of RNA D) pair of DNA
You are interested in a eukaryotic protein involved in immunity, and you are attempting to express this protein in E. coli in order to produce large amounts of the protein
You have identified the gene and place a copy of the gene on a plasmid in E. coli next to a bacterial promoter sequence. You determine that lots of mRNA is made from your gene in your E. coli system, but the protein produced is larger and doesn't have the same properties as the eukaryotic protein you expected. What mistake have you made and how can you fix it? What will be an ideal response?