Suppose the experiment of Meselson and Stahl was performed on a sample of 8 cells, each containing one copy of its circular double-stranded DNA genome, and that had been growing on normal 14N medium. You then grew the cells for 3 generations in medium containing 15N. The outcome would be
A) 8 cells with single-stranded DNA molecules with 14N, and 24 cells with single-stranded DNA molecules with 15N.
B) 16 cells with double-stranded DNA molecules with equal amounts of 14N and 15N, and 48 cells with double-stranded DNA molecules with 15N.
C) 8 cells with double-stranded DNA molecules with equal amounts of 14N and 15N, and 24 cells with double-stranded DNA molecules with 15N.
D) 8 cells with double-stranded DNA molecules with equal amounts of 14N and 15N, and 32 cells with double-stranded DNA molecules with 15N.
E) 65 cells with single-stranded DNA molecules with 15N.
B) 16 cells with double-stranded DNA molecules with equal amounts of 14N and 15N, and 48 cells with double-stranded DNA molecules with 15N.
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A. The incorrect base is recognized because it is methylated. B. The strand containing the misincorporated base is methylated. C. The strand containing the misincorporated base is not methylated. D. The strand containing the misincorporated base is phosphorylated. E. Actually, mismatch repair does lead to mutation about 50% of the time.
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