A microbiologist would like to use a noncompetent genus of streptococcal bacteria, Enterococcus faecalis, as a cloning host to express genes from Streptococcus pneumoniae, which is naturally competent. Is this possible?
A. Yes; electroporation or chemical transformation can be used to make non-competent Streptococcus mutants, from which genes can be inserted into a cloning vector and introduced into Enterococcus.
B. Yes; since the cloning host and the DNA to be introduced are both from bacteria that are streptococci, natural competence in this case is not necessary as long as the appropriate vector is used.
C. Yes; electroporation or chemical transformation can be used to make Enterococcus competent, and then genes from Streptococcus can be introduced via a cloning vector.
D. No; competence factor is an essential protein that enables the uptake of foreign DNA, therefore a cloning host such as Enterococcus that lacks competence factor protein is unable to be transformed by electroporation or chemical transformation.
Answer: C
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