Clostridium difficile is inoculated to two different media. Media X is a rich, nutritious complex growth media. Media Y is a minimal synthetic media containing only the minimum ingredients needed for growth. After 48 hours, which media do you expect to contain a greater proportion of endospores? Explain your reasoning.
b. What is the counterstain used in acid-fast staining?
c. Why can safranin not be used as the counterstain in acid-fast staining?
d. In your own words, explain why some bacterial species produce endospores. What are their purpose?
e. Name two genera of bacteria that produce endospores.
f. What is the primary stain in Schaeffer-Fulton endospore staining?
g. What is the counterstain in Schaeffer-Fulton endospore staining?
h. What is the purpose of steam during Schaeffer-Fulton endospore staining? What do you expect would happen if steam were not used?
a. Media X because it has rich nutrition for endospores to grow and divide.
b. Methylene blue
c. Safranin stains vegetative cells
d. Bacterial species produce endospores as a survival mechanism. The purpose is to grow and divide
e. Clostridium and Bacillus
f. Malachite green
g. Safranin
h. To allow the dye to penetrate the spore coat. If the steam were not used then the dye wouldn’t penetrate the spore coat.
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Fill in the blank(s) with the appropriate word.
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