When PCR amplification of SSU (small subunit ribosomal RNA) yields a population of different PCR products of about the same molecular weight, these fragments can be separated for further analysis by ________.
A. DNA hybridization
B. denaturing gradient gel electrophoresis
C. size exclusion chromatography
D. isoelectric focusing
Answer: B
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The DNA of gene X in humans has been isolated, cloned, and sequenced and is 8000 base pairs in length. From cells that were actively transcribing gene X, a cDNA library was made. In this case, gene X was found to produce from an mRNA that was 4200 bases in length. The most probable explanation of these results is that
A) there was no mRNA in the cells of the mRNA used to make the cDNA library. B) the DNA encoded introns that were excised during mRNA processing. C) a mutation resulted in a large loss of this particular gene. D) reverse transcription of the mRNA isolate was not complete. E) none of the above
In the light-dependent reactions of photosynthesis, an excited electron from photosystem II is passed along an electron
transport chain to ____.
a. NAD+b. NADH c. photosystem I d. oxygen e. water
Morphogens are substances that help control development
Indicate whether the statement is true or false
What are the primary inspiratory muscles?
A) diaphragm and internal intercostals B) external and internal intercostals C) diaphragm and external intercostals D) diaphragm and the external and internal intercostals E) diaphragm and abdominal muscles