Which PCR step causes the denaturation of double-stranded DNA?

A. Add DNA polymerase and nucleotides at 72°C.
B. Cool DNA to between 50°C and 65°C.
C. Heat target DNA to 94°C.
D. Add primers.
E. Repeat the cycle of heating and cooling.


Answer: C

Biology & Microbiology

You might also like to view...

Most lichens are the result of the combination of a fungus and

a. brown algae. b. cyanobacteria. c. green algae. d. red algae

Biology & Microbiology

With minor exceptions, the genetic code ____

a. is the same for all living organisms and viruses b. is specialized so that viruses use a different code than all living organisms c. differs between different organisms and viruses d. is the same for all viruses and for single-celled organisms, but is more complex in multicellular organisms e. has three versions: one for viruses, one for single-celled organisms, and one for multicellular organisms

Biology & Microbiology

Several lineages of reptiles evolved from a common ancestral stem reptile, each adapting to different ways of life.

Answer the following statement true (T) or false (F)

Biology & Microbiology

Gloves, lab coat, eye protection, and/or face shield, plus biosafety cabinets used for all manipulations are the appropriate primary barriers for which level of organism?

A) BSL-1 B) BSL-2 C) BSL-3 D) BSL-4

Biology & Microbiology