The following are steps in using SSU rRNA molecules to develop phylogenetic trees. Place these steps in the correct order.1. Isolate DNA from cells of each organism being tested.2. Amplify the DNA of the SSU rRNA genes of each organism using polymerase chain reaction. 3. Determine the nucleotide sequence of the SSU rRNA genes of each organism.4. Align nucleotide sequences to compare.5. Count the number of nucleotide differences between each pair of sequences and calculate the evolutionary distance.6. Input data into computer and use appropriate software to construct a phylogenetic tree.

Fill in the blank(s) with the appropriate word(s).


1, 5, 3, 4, 6, 2

Biology & Microbiology

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