A microbe is discovered growing near a deep sea thermal vent. When researchers bring a sample up to the surface and try to grow it in a lab at room temperature in a normal incubator, they are unsuccessful. Why?
A.
The pressure isn't the same at sea level as it is on the ocean floor.
B. Oxygen concentrations are very different between the two environments-it's possible the microbe is a strict anaerobe and is poisoned by the air (oxygen) in the lab.
C. Salt concentrations might be different in the media the researchers are attempting to use and the salt water the microbe is used to living in. This might be causing osmotic pressure differences that the microbe can't tolerate.
D.
The temperature is probably different-the thermal vent would be very hot, while these researchers are trying to grow this microbe at room temperature. The enzymes in the cells are probably outside of their normal operating range at room temperature, and therefore nonfunctional.
E. All of the above.
E
You might also like to view...
Transmembrane movement of a substance with a concentration gradient, with no involvement of membrane proteins, is called ________.
Fill in the blank(s) with the appropriate word(s).
A researcher determined that a strain of E. coli is producing a shortened version of a protein required for glucose metabolism. What type of mutation could be responsible for this shorter than normal protein?
A. nonsense mutation B. missense mutation C. silent mutation D. sense mutation E. frameshift mutation
Which of the following has X-linked recessive inheritance?
a. Duchenne muscular dystrophy b. phenylketonuria c. achondroplasia d. sickle-cell anemia e. cystic fibrosis
During library screening, PCR, Southern blotting, and other techniques, binding two pieces of DNA to each other by hydrogen bonding is called ________
A) autoradiography B) hybridization C) DNA ligation D) reverse transcription E) polyadenylation