Positive cooperativity would most likely be observed when a protein is:
A. a single polypeptide with a Kd = 10-10.
B. a single polypeptide with three ligand-binding sites of increasing Kd values.
C. comprised of two identical subunits, each of which binds ligand.
D. comprised of two ligand-binding sites where the Kd for the second site decreases after the first ligand binds.
E. comprised of subunits A and B, where ligand binding to A facilitates ligand binding to B.
E
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