You are working with a mixed population of cells that includes some cells expressing CD95 on their surfaces. Design an experimental protocol using Fluorescence-Activated Cell Sorting (FACS)
to determine how many CD95-expressing cells are dividing at any given time.
What will be an ideal response?
Answer: Stain all the cells with a DNA dye and a fluorescent antibody specific for CD95. Use dual-color flow cytometry to identify cells that have the highest intensity of staining for both DNA and the CD95-specific antibody.
You might also like to view...
Similar families of organisms are next grouped together in the same:a
class b. order c. genus d. phylum e. kingdom
High-energy radiation is often used to treat cancer. The radiation kills the cancerous cells, and the cell cycle checkpoint for DNA repair is overwhelmed. Radiation
a. causes apoptosis at the S and G2 checkpoints. b. inhibits the growth factor at the restriction point. c. blocks DNA replication. d. only causes apoptosis at the tumor site. e. kills all tumor cells without harm to healthy cells.
Roses are classified as
A) angiosperms. B) bryophytes. C) seedless vascular plants. D) gymnosperms. E) ferns.
What is the result of the insertion or deletion of a single base in the coding sequence?
a. frameshift b. an unidentified reading frame c. a new open reading frame d. a silent mutation e. a neutral mutation