A scientist is troubleshooting the synthesis of a cDNA library. The scientist performs both a Northern and a Southern blot. The Northern blot demonstrated the presence of RNA while the Southern blot indicated that no cDNA was present in the sample. What
is likely to be the cause of the failed synthesis of the cDNA library?
A) temperature too cold for annealing B) defective reverse transcriptase
C) proper primers D) too many dNTPs
Answer: B
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Which of the following is not a complementary base pair usually found in DNA molecules?
A. Adenine-uracil B. Guanine-cytosine C. Adenine-thymine D. All of the choices are correct.
A DNA sequence 5? ATGGGGGACACC 3? encodes the amino acids Met Gly Asp Thr. A mutation changes the sequence to 5? ATGAGGGGACACC 3? that encodes the amino acids Met Arg Gly His. What type of mutation has occurred?
A) frameshift mutation B) missense mutation C) silent mutation D) nonsense mutation
Which organelle contains hydrolytic enzymes for the digestion of proteins, lipids, nucleic acids, and polysaccharides?
a. the Golgi complex b. the rough endoplasmic reticulum c. the nucleus d. the lysosome
Protozoa are monophyletic.
Answer the following statement true (T) or false (F)