Which of the following is a disadvantage to expressing genes in E. coli?
A. Bacteria containing recombinant genes rarely will grow in commercial fermenters.
B. Some eukaryotic proteins will not fold properly when expressed in E. coli.
C. Efficient methods exist for transforming DNA into the bacteria but not for extracting DNA.
D. The regulatory sequences that govern gene expression are not well understood and rarely express cloned genes at high levels.
E. Host bacteria are difficult and expensive to grow.
B
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