Which of the following is the best evidence that supercoiling is an intrinsic property of double-stranded DNA molecules?

A. Strand separation during transcription and replication introduces supercoiling.
B. Purified plasmid DNAs are supercoiled even though all proteins are removed.
C. When two strands of an entwined rubber band are pulled apart they will twist into supercoils.
D. Linear fragments of double-stranded DNA are not supercoiled.
E. None of these choices is correct.


B

Biology & Microbiology

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