A manual WBC count is performed using a system that makes a 1:20 dilution of blood. When the cells in the four large corner mm2 of the hemocytometer are counted, the following numbers of cells are obtained: 8, 12, 9, and 11. What would be the next step?
a. Count the cells in all 9 mm2 of the hemocytometer.
b. Redo the dilution of blood using a new 1:20 dilution.
c. Redo the dilution of blood using a 1:100 dilution.
d. Calculate the count using the values from the 4 mm2 and report the result.
ANS: A
This white count appears to be very low, in the neighborhood of 2.0 ´ 109/L [(8 + 12 + 9 + 11) ´ 20/0.4]. To make the cell count more accurate, the number of cells counted must be increased. This can be done by counting the cells in all 9 mm2 of the hemocytometer.
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