Structural differences between Gram-positive and Gram-negative bacteria are observed following the Gram-staining procedure since ________.
A. the thin layer of peptidoglycan in Gram-positive bacteria is dehydrated during the alcohol step, thus acting as an impermeability barrier allowing the stain to wash out
B. the thin layer of peptidoglycan in Gram-negative bacteria is dissolved during the alcohol step, thus acting as an impermeability barrier allowing the crystal violet/iodine complex to be retained
C. the thick layer of peptidoglycan in Gram-positive bacteria is dehydrated during the alcohol step, thus acting as an impermeability barrier retaining the crystal violet/iodine complex
D. the thick layer of peptidoglycan in Gram-negative bacteria is dehydrated during the alcohol step, thus acting as an impermeability barrier retaining the purple stain
Answer: C
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Fill in the blank(s) with the appropriate word(s).
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