You have a segment of DNA that contains the following sequence:
5?-GGACTAGACAATAGGGACCTAGAGATTCCGAAA-3?
3?-CCTGATCTGTTATCCCTGGATCTCTAAGGCTTT-5?
You know that the RNA transcribed from this segment contains the following sequence:
5?-GGACUAGACAAUAGGGACCUAGAGAUUCCGAAA–3?
Which of the following choices best describes how transcription occurs?
(a) the top strand is the template strand; RNA polymerase moves along this strand from 5? to 3?
(b) the top strand is the template strand; RNA polymerase moves along this strand from 3? to 5?
(c) the bottom strand is the template strand; RNA polymerase moves along this strand from 5? to 3?
(d) the bottom strand is the template strand; RNA polymerase moves along this strand from 3? to 5?
(d) The bottom strand can hybridize with the RNA molecule and thus is the template strand. The polymerase moves along the DNA in a 3?-to-5? direction, because the RNA nucleotides are joined in a 5?-to-3? polarity.
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