Rank the following steps in the correct order to describe the process of seamless cloning.1. DNA to be inserted is amplified by PCR and contains 5' sequences that match the vector's junction site2. Vector is linearized and mixed with the amplified DNA3. T5 exonuclease cleaves the 5' ends of all fragments leaving 3' sticky ends4. The vector and amplified DNA hybridize at their complementary 3' sticky ends5. DNA polymerase extends the 3' ends of the vector and inserted DNA strands6. DNA ligase seals the gaps between vector and insert to close the recombinant circular molecule
Fill in the blank(s) with the appropriate word(s).
2, 3, 1, 5, 4, 6
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A. NADH. B. NAD. C. NAD-. D. NADP.
Tuberculin skin testing
A. uses a purified protein filtrate of Mycobacterium tuberculosis. B. will be positive in active TB. C. involves the injection of PPD intradermally. D. will be positive if person has had previous exposure. E. All of the choices are correct.
The specific parts of foreign proteins that the immune system recognizes and "remembers" are called ________
A) receptor sites B) binding sites C) antibody sites D) antigenic sites E) None of the above.
All of the following are major functions of roots EXCEPT
A) carrying out photosynthesis. B) interacting with soil fungi. C) transporting water and minerals. D) storing excess sugars. E) producing hormones.