You stain a cell with a dye that reacts with an enzyme found in the lysosome. You would also expect to see this dye in the:

Question

A.  nucleus.
B.  mitochondria.
C.  endoplasmic reticulum.
D.  cytoplasm.

Clarify Question
· What is the key concept addressed by the question?
· What type of thinking is required?
· What key words does the question contain and what do they mean?
 
Gather Content
· What do you know about lysosome proteins? How does it relate to the question?
 
Consider Possibilities
· What other information is related to the question? Which information is most useful?

Choose Answer
· Given what you now know, what information and/or problem solving approach is most likely to produce the correct answer?

Reflect on Process
· Did your problem-solving process lead you to the correct answer? If not, where did the process break down or lead you astray? How can you revise your approach to produce a more desirable result?

Answer 1

C.  endoplasmic reticulum.

Clarify Question
· What is the key concept addressed by the question?
        o The question asks about an enzyme in the lysosome.
· What type of thinking is required?
        o You are being asked to apply your knowledge of how proteins move into the lysosome to explain the results of an experiment.
· What key words does the question contain and what do they mean?
        o Dye that reacts with an enzyme found in the lysosome – there are specific stains that bind one protein and not another.
 
Gather Content
· What do you know about lysosome proteins? How does it relate to the question?
        o Enzymes from the lysosome are first made in the endoplasmic reticulum, where they move through the Golgi, and then on to the lysosome.
 
Consider Possibilities
· What other information is related to the question? Which information is most useful?
        o Proteins are not made in the nucleus.
        o Proteins made in the cytoplasm remain in the cytoplasm.
        o Some proteins are made in the mitochondria, but remain there.

Choose Answer
· Given what you now know, what information and/or problem solving approach is most likely to produce the correct answer?
        o Proteins that are secreted or transported into organelles like the lysosome are first made in the endoplasmic reticulum.

Reflect on Process
· Did your problem-solving process lead you to the correct answer? If not, where did the process break down or lead you astray? How can you revise your approach to produce a more desirable result?
        o This question asked you to apply your understanding of protein secretion to explain where an enzyme in the lysosome was made. If you got the correct answer, great job! If you got an incorrect answer, where did the process break down? Did you recall that proteins made in the endoplasmic reticulum are transported to other organelles like the lysosome?  Did you think that proteins made in the cytoplasm could cross a membrane and enter an organelle like the lysosome?