If FRAP is performed on two membranes, one at 25 ºC and the other at 40 ºC, which one of the following results would most likely be observed?
A. Only the membrane at the higher
temperature would be capable of recovering
fluorescence in the bleached area.
B. Only the membrane at the lower
temperature would be capable of recovering
fluorescence in the bleached area.
C. Both membranes would recover
fluorescence in the bleached area, but the
membrane at the colder temperature would
recover more rapidly.
D. Both membranes would recover
fluorescence in the bleached area, but the
membrane at the warmer temperature would
recover more rapidly.
E. Both membranes would recover
fluorescence in the bleached area, and at the
same rate.
Answer Key: D
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