What experimental evidence suggests that the N-terminal tails of histones participate in the formation of higher-order chromatin structure?
a) The tails cause histones to denature.
b) Chromatin fibers prepared with H4 histones lacking their tails cannot fold into higher-order fibers.
c) Chromatin fibers prepared with H4 histones possessing longer tails cannot fold into higher-order fibers.
d) The tails when acetylated cause the dissolution of the 30-nm fibers.
e) Chromatin fibers lacking tails are shorter.
Ans: c) Chromatin fibers prepared with H4 histones possessing longer tails cannot fold into higher-order fibers.
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