Two 6-base sequences are present in bacterial promoters: TATAAT (located 10 nt upstream from the start site) and TTGACA (located 35 nt upstream from the start site). What is the significance of the fact that these two base sequences are different?
A. Binding sites for both the holoenzyme and ATP are provided.
B. It allows RNA polymerase to distinguish between the template strand and the coding strand of the DNA molecule.
C. The transcription bubble can be properly formed.
D. Binding sites for both the core polymerase and holoenzyme are provided.
E. Both the location of the start site and the direction of transcription can be established.
Answer: E
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