Why is a "no DNA" control used when performing molecular tests using the polymerase chain reaction (PCR)?
a. To ensure that the targeted DNA is amplified
b. To ensure that nontargeted DNA is negative
c. To ensure that the targeted DNA containing the mutation is positive
d. To ensure that no DNA cross-contamination of the samples, controls, or reagents occurs
ANS: D
Contamination of reagents, controls, and samples with extraneous DNA from the environment is possible when performing molecular testing. Thus a no DNA control must be run along with the controls and samples to validate the analysis.
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