In the disk diffusion method of antimicrobial susceptibility testing, what is the standard medium used for testing most bacterial organisms?
a. Mueller-Hinton broth
b. Mueller-Hinton agar
c. Brain-heart infusion agar
d. Brucella broth with 5% lysed horse blood
B
Mueller-Hinton (MH) agar is the standard agar base medium used for testing most bacterial or-ganisms in the disk diffusion method of antimicrobial susceptibility testing. Cation-adjusted MH broth (CAMHB) is used in the broth dilution susceptibility testing of most bacteria. Brucella blood agar or Wilkins Chalgren agar is used for anaerobic susceptibility testing by agar dilution and Brucella broth with LHB is used for broth dilution susceptibility testing against Bacterioides fragilis. Brain-Heart infusion (BHI) agar is used for vancomycin resistance screening for staph-lococci and enterococci.
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