Describe a DNA microarray setup for assaying mutations associated with a hypothetical genetic disease
What will be an ideal response?
Normal and mutant gene sequences associated with the disease could be affixed to the array in known positions. DNA from a patient and a known nonmutant individual could be separately isolated and labeled (or mRNA could be used, making cDNA for labeling with reverse transcription). Both are applied to the microarray, and whichever labeled fragments that bind with the DNA fragments on the array will colorimetrically reveal which alleles the patient possesses.
You might also like to view...
Virions infecting some bacteria possess the enzyme ________ that makes a small hole in the bacterial cell wall, allowing the viral nucleic acid to enter
A) peptidoglycanase B) infectase C) lysozyme D) nuclease
The bones in the forelimbs of a mammal
a. can often be traced to a common ancestor. b. offer no evidence to support the theory of evolution. c. perform the same function no matter which species they are in. d. may exhibit either analogy or homology but not both when compared to the forelimb of another animal. e. show convergence with some invertebrate structures.
Plant stem cells are able to do which of the following?
A. They grow into leaves. B. They are mature cells. C. They are located in the developed part of the vascular tissue. D. They are undifferentiated cells that can produce new tissues. E. They are mostly found at the tip of leaves.
Salamanders are members of the order:a
Apoda. b. Caudata. c. Anura.
d. Tetrapoda. e. Squamata.