The fluorochrome method of staining Mycobacterium:

a. is less sensitive than the conventional carbolfuchsin stains.
b. enables the smear to be initially examined at lower magnifications.
c. results in a lower yield of positive smears.
d. increases the amount of time needed for examining smears.


B
The fluorochrome stain is more sensitive than the conventional carbolfuchsin stains; the fluores-cent bacilli stand out brightly against the background and can be initially examined at lower magnifications. More fields can be visualized in a short period. In addition, a positive fluorescent smear may be restained by the conventional Ziehl-Neelsen or Kinyoun's procedure, thereby sav-ing the time needed to make a fresh smear.

Biology & Microbiology

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