In 1944, Avery, MacLeod, and McCartney performed transformation experiments using live, harmless bacteria and extracts from virulent bacteria treated with various enzymes. Which of the following were used and why?

A) Proteases and RNases to rule out protein and RNA as the transforming factors.
B) Lipase to rule out lipoproteins as the transforming factor.
C) Kinase to show that transformation is phosphorylation dependent.
D) ATPase to show that transformation is not dependent on ATP.


Answer: A) Proteases and RNases to rule out protein and RNA as the transforming factors.

Biology & Microbiology

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