The enhanced selectivity of tandem affinity purification (TAP) is due to:
A. two different tags?calmodulin-binding protein and protein A?that are fused to the protein of interest and allow consecutive rounds of affinity chromatography.
B. the presence of a TEV cleavage site in the fusion protein. Cleavage of this site further purifies the protein by cleaving of the protein A tag.
C. the fusion of a Gal4p DNA binding domain to the protein along with protein A. These two tags allow for purification of the fusion protein.
D. the calmodulin-binding protein tag, which binds strongly to calmodulin.
E. All of these choices contribute to the enhanced selectivity.
A
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