A definitive identification of a pathogen typically includes:

a. a description of the colonial and micro-scopic morphology.
b. a description of the colonial and bio-chemical reactions.
c. a confirmatory set of reactions that corre-lates to the preliminary characteristics.
d. a confirmatory set of reactions and anti-biogram for all organisms.


C
The skilled microbiologist is able to complete a set of confirmatory reactions from isolated pure colonies either using a manual set of reactions or automated panel. The results of the reactions should correlate with the preliminary microscopic characteristics as well as with an organism or pathogen that may be present in the type of specimen submitted. It is important for the microbi-ologist to be able to distinguish if the information is correct and correlates with the results prior to reporting a definitive identification to the physician. Depending on the source and identified organism, not all work-ups require the reporting or completion of an antimicrobial susceptibility panel.

Biology & Microbiology

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