How is initiation at oriC regulated in bacteria?
A. SeqA binds to hemimethylated DNA and sequesters the newly replicated origin.
B. DnaA-ADP, formed when DnaA hydrolyzes its ATP, cannot maintain the destabilization of the origin so the closed complex reforms.
C. The Hda protein binds the ? clamp causing DnaA to hydrolyze its ATP.
D. RNA polymerase activity produces superhelical twisting that promotes melting of the origin by DnaA.
E. All of these choices are correct.
E
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A compound known as X-gal is widely used in molecular genetic research. When wild-type (normal) E. coli is grown on medium containing X-gal, the bacterial colonies turn blue. In contrast, when lacZ mutants are grown on medium containing X-gal
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A nuclear envelope has four of the following characteristics. Which is the exception?
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