At 4:00 p.m. a closed flask of sterile broth is inoculated with 10,000 cells. The lag phase lasts 1 hour. At 9:00 p.m. the culture enters stationary phase with a population of approximately 41 million cells. At what time is the population half maximal?
A. 7:20 p.m.
B. 6:30 p.m.
C. 8:20 p.m.
D. 8:40 p.m.
Answer: D
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The best possible analogy available below for the way in which variable (V), diversity (D), and joining (J) antibody gene segments get put together to create the diversity possible in hypervariable regions is
A. to think of the various segments as a deck of cards-when you get dealt a hand of five cards, you have a very high likelihood of getting a diferent hand every time. The quality of the hand you have dealt will dictate whether you have a "winning" hand (capable of binding to antigenic epitopes). B. to think of the various segments as the pieces of a house-you need a strong foundation first (the joining segments), followed by a frame (the diversity segments), then the interior walls (the variable segments) before the structure is complete. C. to think of the various segments as building a highway-you need to prepare the area first by clearing a path (the joining segments do this), then grade/slope the area (the diversity segments) before you can finally lay down the asphalt (the variable segments). D. to think of the various segments as a bingo game-each segment is randomly selected, but you're going to need one of each V, D, and J to form a functional molecule. The "right" combination varaies depending on which antigen is eventually going to be binding to the molecule (i.e., your bingo card would be the eventual antigen, and the random calling out of the number/letter combinations would be the forming of the VDJ hypervariable region).
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