The concentration protocol of the ova and parasite (O&P) examination:
a. requires freshly passed, unpreserved stool.
b. is designed to allow the detection of pro-tozoan cysts, coccidian oocysts, micro-sporidial spores, and helminth eggs and larvae.
c. is microscopically examined at low, high dry, and oil immersion magnifications.
d. is designed to facilitate the identification of intestinal protozoa.
B
The O&P examination is made up of three separate protocols: (1) the direct wet mount, (2) the concentration, and (3) the permanent stained smear. The second part of the O&P examination is the concentration, which is designed to facilitate the recovery of protozoan cysts, coccidian oo-cysts, microsporidial spores, and helminth eggs and larvae. Both flotation and sedimentation methods are available; the most common procedure is the formalin–ethyl acetate sedimentation method (formerly called the formalin-ether method). The concentrated specimen is examined as a wet preparation, with or without iodine, using low and high dry magnifications (100, 400) as indicated for the direct wet smear examination.
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