How does an RFLP help identify sickle-cell anemia?

What will be an ideal response?


The normal globin gene is associated with three DdeI sites. The mutation that causes sickle-cell anemia changes one of those sites, leaving only two. This can be detected by PCR amplifying the globin gene and digesting with the DdeI restriction enzyme. A normal person yields two bands, while a person with sickle-cell anemia yields only one larger band.

Biology & Microbiology

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The human knee-jerk reflex requires an intact _____

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Biology & Microbiology

Charles Darwin and his son experimented with which plant growth response?

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Biology & Microbiology

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Biology & Microbiology